Rue is a cryptic, but significant NPC (and in the Equinox Update, a temporarily playable character) located in a room in the back alley of Refuge, to the right of the Lamplighter's house. She also features in one of the three visions Niko can encounter during their travels.

She is found in a strange, small area in the maze-like Alley. There is a large tree here that appears to be be shone on by sunbeams. Though these could be city lights trickling down from above, the tree itself seems out of place given it should be in much poorer condition without the Sun.

Rue, similar to Niko, is kindhearted and caring, talking to Niko in a friendly manner even though they usually don't remember her. After the Equinox Update, she is also shown to be obedient and loyal as she followed the Author's written advice despite such likely causing her loneliness.