Yuri Tsukikage is seen in Heartcatch Pretty Cure!, though is considered the fourth Cure to join the heroines, and throughout most of the first half of the season, she is clouded in mystery. She appears in the dreams of Tsubomi,Erika and Itsuki before they become Pretty Cure. In the series, she is the first Cure to make an appearance, her identity being Cure Moonlight. Despite being a Pretty Cure before the series started, she can't become one now because in the battle with Dark Cure, her partner, Cologne, sacrificed himself to save her and ever since she went through this painful experience of seeing her friend die for her, her heart Flower wilted completely and her heart seed broke. As a result, she was incapable of becoming a Pretty Cure. But soon, she became a Pretty Cure again and revived her legacy as Cure Moonlight after she met Colonge again as a spirit. After reasoning her and hearing her friends are in danger, she begged the Tree of hearts to let her become a Pretty Cure again and also seeing Colonge again, her Heart Flower revived too.

Yuri is calm and composed, well known for her cool attitude and her frequently chart-topping exam scores at school. She cares for little and is initially cold towards Tsubomi and Erika. After losing Cologne she became quiet and withdrawn, fearful of causing others to feel burdened by her or risk losing someone else she cherishes. She believed it would be impossible for her to become a Pretty Cure again and was deeply saddened. Before this happened, she was shown to be overly confident and bold. She wouldn't listen to Cologne when she believed she didn't need a partner for help until Cologne died for her.

Her true personality is one of contentment and maturity. She can be stubborn when she sets her mind to something but begins to open up as she spends more time with the group. She shows a more friendly side to herself by this point. Although generally mature, she can sometimes be oblivious to other's romantic feelings for her.